PUBLICATIONS

All students and postdocs in Huang lab are underlined below.

2017

Coupling between protein stability and catalytic activity determines pathogenicity of G6PD mutations
Cunningham AD, Colavin A, Huang KC, Mochly-Rosen Daria. Cell Reports 18 2592-2599 (2017).

GTPase activity-coupled treadmilling of the bacterial tubulin FtsZ directs septal cell-wall synthesis
Yang X, Lyu Z*, Miguel A*, McQuillen R, Huang KC, Xiao J, Science 355 744-747 (2017).
*Equal Contributors Co-corresponding authors

Emergent Phototactic Responses of Cyanobacteria under Complex Light Regimes
RMW Chau, D Bhaya, Huang KC. mBio 8 e02330-16 (2017).
Co-corresponding authors

Rapid, precise quantification of bacterial cellular dimensions across a genomic-scale knockout library
Ursell T*, Lee*, Shiomi D, Shi H, Tropini C, Monds RD, Colavin A, Billings G, Bhaya-Grossman I, Broxton M, Huang BE, Niki H, **KC Huang**, BMC Biology 15 17 (2017). \*Co-first authors

Staying in touch while on the go
Huang KC, Cell 168 15-17 (2017).

Long-term microfluidic tracking of coccoid cyanobacterial cells reveals robust control of division timing
Yu F, Willis L, Chau RM, Zambon A, Horowitz MA, Bhaya D, Huang KC, Quake SR. BMC Biology 15 11 (2017).
Co-corresponding authors

Strain Library Imaging Protocol: high-throughput, automated single-cell microscopy for large bacterial collections arrayed on multiwell plates, Shi H*, Colavin A*, Lee TK, Huang KC, Nature Protocols 12 429-438 (2017).
*Co-first authors

A Periplasmic Polymer Curves Vibrio cholerae and Promotes Pathogenesis
Bartlett TM, Bratton BP, Duvshani A, Miguel A, Sheng Y, Martin NR, Nguyen JP, Persat A, Desmarais SM, VanNieuwenhze MS, Huang KC. Cell doi: 10.1016/j.cell.2016.12.019 168(1):172-85. Epub 2017 Jan 12.

2016

Cell size and growth regulation in the Arabidopsis thaliana apical stem cell niche
Willis L, Refahia Y, Wightmana R, Landreina B, Telesa J, Huang KC, Meyerowitz EM, Jönssona H. Proceedings of the National Academy of Sciences 113.51 doi: 10.1073/pnas.1616768113 Epub 2016 Nov 3.

Single-molecule imaging reveals modulation of cell wall synthesis dynamics in live bacterial cells.
Lee TK, Meng K, Shi H, Huang KC. Nature Communications 2016 Sep 8. doi: 10.1038/ncomms13170. Epub 2016 Oct 24.

FtsZ-Dependent Elongation of a Coccoid Bacterium.
Pereira AR, Hsin J, Król E, Tavares AC, Flores P, Hoiczyk E, Ng N, Dajkovic A, Brun YV, VanNieuwenhze MS, Roemer T, Carballido-Lopez R, Scheffers D, Huang KC, Pinho, MG. MBio 2016 Sep 6;7(5). doi: 10.1128/mBio.00908-16.

Mechanical Genomics Identifies Diverse Modulators of Bacterial Cell Stiffness.
Auer GK, Lee TK, Rajendram M, Cesar S, Miguel A, Huang KC, Weibel DB. Cell Syst. 2(6):402-11. doi: 10.1016/j.cels.2016.05.006. Epub 2016 Jun 16.
Co-corresponding authors

A Comprehensive, CRISPR-based Functional Analysis of Essential Genes in Bacteria.
Peters JM*, Colavin A*, Shi H*, Czarny TL, Larson MH, Wong S, Hawkins JS , Lu CHS, Koo B-M , Marta E, Shiver AL, Whitehead EH, Weissman JS, Brown ED, Qi LS, Huang KC, Gross CA. Cell. 2016 165 1-14 (2016).
*Co-first authors Co-corresponding authors

Disruption of lipid homeostasis in the Gram-negative cell envelope activates a novel cell death pathway.
Sutterlin HA*, Shi H*, May KL, Miguel A, Khare S, Huang KC, Silhavy TJ.
Proc Natl Acad Sci U S A. 2016 Mar 15;113(11):E1565-74. doi: 10.1073/pnas.1601375113. Epub 2016 Feb 29.
*Co-first authors Co-corresponding authors

2015

The effect of microbial colonization on the host proteome varies by gastrointestinal location.
Lichtman JS, Alsentzer E, Jaffe M, Sprockett D, Masutani E, Ikwa E, Fragiadakis GK, Clifford D, Huang BE, Sonnenburg JL, Huang KC, Elias JE.
ISME J. 2016 May;10(5):1170-81. doi: 10.1038/ismej.2015.187. Epub 2015 Nov 17.
Co-corresponding authors

Cytoskeletal Network Morphology Regulates Intracellular Transport Dynamics.
Ando D, Korabel N, Huang KC, Gopinathan A.
Biophys J. 2015 Oct 20;109(8):1574-82. doi: 10.1016/j.bpj.2015.08.034.
Co-corresponding authors

Physics of Intracellular Organization in Bacteria.
Wingreen NS, Huang KC.
Annu Rev Microbiol. 2015;69:361-79. doi: 10.1146/annurev-micro-091014-104313. Review.

High-throughput, Highly Sensitive Analyses of Bacterial Morphogenesis Using Ultra Performance Liquid Chromatography.
Desmarais SM, Tropini C, Miguel A, Cava F, Monds RD, de Pedro MA, Huang KC.
J Biol Chem. 2015 Dec 25;290(52):31090-100. doi: 10.1074/jbc.M115.661660. Epub 2015 Oct 14.

Quantitative Imaging of Gut Microbiota Spatial Organization.
Earle KA*, Billings G*, Sigal M, Lichtman JS, Hansson GC, Elias JE, Amieva MR, Huang KC, Sonnenburg JL.
Cell Host Microbe. 2015 Oct 14;18(4):478-88. doi: 10.1016/j.chom.2015.09.002. Epub 2015 Oct 1.
*Co-first authors

Applications of imaging for bacterial systems biology.
Huang KC.
Curr Opin Microbiol. 2015 Oct;27:114-20. doi: 10.1016/j.mib.2015.08.003. Epub 2015 Sep 8. Review.

Nanoengineering: Super symmetry in cell division.
Huang KC.
Nat Nanotechnol. 2015 Aug;10(8):655-6. doi: 10.1038/nnano.2015.148.

How Does the Xenopus laevis Embryonic Cell Cycle Avoid Spatial Chaos?
Gelens L, Huang KC, Ferrell JE Jr.
Cell Rep. 2015 Aug 4;12(5):892-900. doi: 10.1016/j.celrep.2015.06.070. Epub 2015 Jul 23.

The bacterial tubulin FtsZ requires its intrinsically disordered linker to direct robust cell wall construction.
Sundararajan K, Miguel A, Desmarais SM, Meier EL, Huang KC, Goley ED.
Nat Commun. 2015 Jun 23;6:7281. doi: 10.1038/ncomms8281.

Coordination of peptidoglycan synthesis and outer membrane constriction during Escherichia coli cell division.
Gray AN, Egan AJ, Van’t Veer IL, Verheul J, Colavin A, Koumoutsi A, Biboy J, Altelaar AF, Damen MJ, Huang KC, Simorre JP, Breukink E, den Blaauwen T, Typas A, Gross CA, Vollmer W.
Elife. 2015 May 7;4. doi: 10.7554/eLife.07118.

Bacterial division. Mechanical crack propagation drives millisecond daughter cell separation in Staphylococcus aureus.
Zhou X*, Halladin DK*, Rojas ER*, Koslover EF, Lee TK, Huang KC, Theriot JA.
Science. 2015 May 1;348(6234):574-8. doi: 10.1126/science.aaa1511.
*Co-first authors

Maintenance of motility bias during cyanobacterial phototaxis.
Chau RM, Ursell T, Wang S, Huang KC, Bhaya D.
Biophys J. 2015 Apr 7;108(7):1623-32. doi: 10.1016/j.bpj.2015.01.042.
Co-corresponding authors

Structural basis for the geometry-driven localization of a small protein.
Gill RL Jr*, Castaing JP*, Hsin J*, Tan IS, Wang X, Huang KC, Tian F, Ramamurthi KS.
Proc Natl Acad Sci U S A. 2015 Apr 14;112(15):E1908-15. doi: 10.1073/pnas.1423868112. Epub 2015 Mar 30.
*Co-first authors Co-corresponding authors

Variations in the binding pocket of an inhibitor of the bacterial division protein FtsZ across genotypes and species.
Miguel A, Hsin J, Liu T, Tang G, Altman RB, Huang KC.
PLoS Comput Biol. 2015 Mar;11(3):e1004117. doi: 10.1371/journal.pcbi.1004117.

2014

Systematic perturbation of cytoskeletal function reveals a linear scaling relationship between cell geometry and fitness.
Monds RD, Lee TK, Colavin A, Ursell T, Quan S, Cooper TF, Huang KC.
Cell Rep. 2014 Nov 20;9(4):1528-37. doi: 10.1016/j.celrep.2014.10.040. Epub 2014 Nov 13.

Principles of bacterial cell-size determination revealed by cell-wall synthesis perturbations.
Tropini C, Lee TK, Hsin J, Desmarais SM, Ursell T, Monds RD, Huang KC.
Cell Rep. 2014 Nov 20;9(4):1520-7. doi: 10.1016/j.celrep.2014.10.027. Epub 2014 Nov 6.
Co-corresponding authors

The contractile ring coordinates curvature-dependent septum assembly during fission yeast cytokinesis.
Zhou Z, Munteanu EL, He J, Ursell T, Bathe M, Huang KC, Chang F.
Mol Biol Cell. 2015 Jan 1;26(1):78-90. doi: 10.1091/mbc.E14-10-1441. Epub 2014 Oct 29.

How and why cells grow as rods.
Chang F, Huang KC.
BMC Biol. 2014 Aug 2;12:54. doi: 10.1186/s12915-014-0054-8. Review.
Co-corresponding authors

De novo morphogenesis in L-forms via geometric control of cell growth.
Billings G, Ouzounov N, Ursell T, Desmarais SM, Shaevitz J, Gitai Z, Huang KC.
Mol Microbiol. 2014 Sep;93(5):883-96. doi: 10.1111/mmi.12703. Epub 2014 Jul 23.
Co-corresponding authors

Response of Escherichia coli growth rate to osmotic shock.
Rojas ER, Theriot JA, Huang KC.
Proc Natl Acad Sci U S A. 2014 May 27;111(21):7807-12. doi: 10.1073/pnas.1402591111. Epub 2014 May 12.
Co-corresponding authors

Rod-like bacterial shape is maintained by feedback between cell curvature and cytoskeletal localization.
Ursell TS, Nguyen J, Monds RD, Colavin A, Billings G, Ouzounov N, Gitai Z, Shaevitz JW, Huang KC.
Proc Natl Acad Sci U S A. 2014 Mar 18;111(11):E1025-34. doi: 10.1073/pnas.1317174111. Epub 2014 Feb 18.

Effects of polymerization and nucleotide identity on the conformational dynamics of the bacterial actin homolog MreB.
Colavin A, Hsin J, Huang KC.
Proc Natl Acad Sci U S A. 2014 Mar 4;111(9):3585-90. doi: 10.1073/pnas.1317061111. Epub 2014 Feb 18.

A dynamically assembled cell wall synthesis machinery buffers cell growth.
Lee TK, Tropini C, Hsin J, Desmarais SM, Ursell TS, Gong E, Gitai Z, Monds RD, Huang KC.
Proc Natl Acad Sci U S A. 2014 Mar 25;111(12):4554-9. doi: 10.1073/pnas.1313826111. Epub 2014 Feb 18.
Co-corresponding authors

Isolation and preparation of bacterial cell walls for compositional analysis by ultra performance liquid chromatography.
Desmarais SM, Cava F, de Pedro MA, Huang KC.
J Vis Exp. 2014 Jan 15;(83):e51183. doi: 10.3791/51183.

2013

Motility enhancement through surface modification is sufficient for cyanobacterial community organization during phototaxis.
Ursell T*, Chau RM*, Wisen S*, Bhaya D, Huang KC.
PLoS Comput Biol. 2013;9(9):e1003205. doi: 10.1371/journal.pcbi.1003205. Epub 2013 Sep 5.
*Co-first authors Co-corresponding authors

The role of hydrolases in bacterial cell-wall growth.
Lee TK, Huang KC.
Curr Opin Microbiol. 2013 Dec;16(6):760-6. doi: 10.1016/j.mib.2013.08.005. Epub 2013 Sep 12. Review.

FtsZ protofilaments use a hinge-opening mechanism for constrictive force generation.
Li Y, Hsin J*, Zhao L*, Cheng Y*, Shang W, Huang KC, Wang HW, Ye S.
Science. 2013 Jul 26;341(6144):392-5. doi: 10.1126/science.1239248.
*Co-first authors

Optimal dynamics for quality control in spatially distributed mitochondrial networks.
Patel PK, Shirihai O, Huang KC.
PLoS Comput Biol. 2013;9(7):e1003108. doi: 10.1371/journal.pcbi.1003108. Epub 2013 Jul 11.

Dimer dynamics and filament organization of the bacterial cell division protein FtsA.
Hsin J, Fu R, Huang KC.
J Mol Biol. 2013 Nov 15;425(22):4415-26. doi: 10.1016/j.jmb.2013.07.016. Epub 2013 Jul 17.

Design of High-Specificity Nanocarriers by Exploiting Non-Equilibrium Effects in Cancer Cell Targeting.
Tsekouras K, Goncharenko I, Colvin ME, Huang KC, Gopinathan A.
PLoS One. 2013 Jun 26;8(6):e65623. doi: 10.1371/journal.pone.0065623. Print 2013.
Co-corresponding authors

Biological consequences and advantages of asymmetric bacterial growth.
Kysela DT, Brown PJ, Huang KC, Brun YV.
Annu Rev Microbiol. 2013;67:417-35. doi: 10.1146/annurev-micro-092412-155622. Epub 2013 Jun 26. Review.

Mechanical consequences of cell-wall turnover in the elongation of a Gram-positive bacterium.
Misra G, Rojas ER, Gopinathan A, Huang KC.
Biophys J. 2013 Jun 4;104(11):2342-52. doi: 10.1016/j.bpj.2013.04.047.

Peptidoglycan at its peaks: how chromatographic analyses can reveal bacterial cell wall structure and assembly.
Desmarais SM, De Pedro MA, Cava F, Huang KC.
Mol Microbiol. 2013 Jul;89(1):1-13. doi: 10.1111/mmi.12266. Epub 2013 Jun 3. Review.
Co-corresponding authors

2012

The molecular origins of chiral growth in walled cells.
Huang KC, Ehrhardt DW, Shaevitz JW.
Curr Opin Microbiol. 2012 Dec;15(6):707-14. doi: 10.1016/j.mib.2012.11.002. Epub 2012 Nov 26. Review.
Co-corresponding authors

Analysis of surface protein expression reveals the growth pattern of the gram-negative outer membrane.
Ursell TS*, Trepagnier EH*, Huang KC, Theriot JA.
PLoS Comput Biol. 2012;8(9):e1002680. doi: 10.1371/journal.pcbi.1002680. Epub 2012 Sep 27.
*Co-first authors Co-corresponding authors

Physical constraints on the establishment of intracellular spatial gradients in bacteria.
Tropini C, Rabbani N, Huang KC.
BMC Biophys. 2012 Aug 29;5:17. doi: 10.1186/2046-1682-5-17.

Interplay between the localization and kinetics of phosphorylation in flagellar pole development of the bacterium Caulobacter crescentus.
Tropini C, Huang KC.
PLoS Comput Biol. 2012;8(8):e1002602. doi: 10.1371/journal.pcbi.1002602. Epub 2012 Aug 2.

Posttranslational acetylation of a-tubulin constrains protofilament number in native microtubules.
Cueva JG, Hsin J, Huang KC, Goodman MB.
Curr Biol. 2012 Jun 19;22(12):1066-74. doi: 10.1016/j.cub.2012.05.012. Epub 2012 May 31.

Nucleotide-dependent conformations of FtsZ dimers and force generation observed through molecular dynamics simulations
Hsin J, Gopinathan A, Huang KC. Proc Natl Acad Sci U S A. 2012 Jun 12;109(24):9432-7. doi: 10.1073/pnas.1120761109. Epub 2012 May 30.

Measuring the stiffness of bacterial cells from growth rates in hydrogels of tunable elasticity.
Tuson HH*, Auer GK*, Renner LD, Hasebe M, Tropini C, Salick M, Crone WC, Gopinathan A, Huang KC, Weibel DB.
Mol Microbiol. 2012 Jun;84(5):874-91. doi: 10.1111/j.1365-2958.2012.08063.x. Epub 2012 May 2.
*Co-first authors Co-corresponding authors

Helical insertion of peptidoglycan produces chiral ordering of the bacterial cell wall.
Wang S, Furchtgott L, Huang KC, Shaevitz JW.
Proc Natl Acad Sci U S A. 2012 Mar 6;109(10):E595-604. doi: 10.1073/pnas.1117132109. Epub 2012 Feb 17.
Co-corresponding authors

2011

Resolution limits of optical microscopy and the mind.
Ursell TS and Huang, KC.
Biomed Comp Rev7 27 (2011).

The bacterial actin MreB rotates, and rotation depends on cell-wall assembly.
van Teeffelen S, Wang S, Furchtgott L, Huang KC, Wingreen NS, Shaevitz JW, Gitai Z.
Proc Natl Acad Sci U S A. 2011 Sep 20;108(38):15822-7. doi: 10.1073/pnas.1108999108. Epub 2011 Sep 8.

Conformational changes, diffusion and collective behavior in monomeric kinesin-based motility.
Huang KC, Vega C, Gopinathan A.
J Phys Condens Matter. 2011 Sep 21;23(37):374106. doi: 10.1088/0953-8984/23/37/374106. Epub 2011 Aug 23.

Does the potential for chaos constrain the embryonic cell-cycle oscillator?
McIsaac RS*, Huang KC*, Sengupta A, Wingreen NS.
PLoS Comput Biol. 2011 Jul;7(7):e1002109. doi: 10.1371/journal.pcbi.1002109. Epub 2011 Jul 14.
*Co-first authors

Mechanics of membrane bulging during cell-wall disruption in gram-negative bacteria.
Daly KE, Huang KC, Wingreen NS, Mukhopadhyay R.
Phys Rev E Stat Nonlin Soft Matter Phys. 2011 Apr;83(4 Pt 1):041922. Epub 2011 Apr 25.

Mechanisms for maintaining cell shape in rod-shaped Gram-negative bacteria.
Furchtgott L, Wingreen NS, Huang KC.
Mol Microbiol. 2011 Jul;81(2):340-53. doi: 10.1111/j.1365-2958.2011.07616.x. Epub 2011 Apr 18.

Bilayer-mediated clustering and functional interaction of MscL channels.
Grage SL, Keleshian AM, Turdzeladze T, Battle AR, Tay WC, May RP, Holt SA, Contera SA, Haertlein M, Moulin M, Pal P, Rohde PR, Forsyth VT, Watts A, Huang KC, Ulrich AS, Martinac B.
Biophys J. 2011 Mar 2;100(5):1252-60. doi: 10.1016/j.bpj.2011.01.023.
Co-corresponding authors

Spatial gradient of protein phosphorylation underlies replicative asymmetry in a bacterium
Chen YE*, Tropini C*, Huang KC, Laub MT.
Proc Nat Acad Sci USA108 1052-1057 (2011). Selected for Feb 1, 2011 issue of Virtual Journal of Biological Physics Research.
*Co-first authors Co-corresponding authors

2010

Dynamic SpoIIIE assembly mediates septal membrane fission during Bacillus suptilis sporulation.
Fleming TC, Shin JY, Lee SH, Becker E, Huang KC, Bustamante C, Pogliano K.
Genes Dev. 2010 Jun 1;24(11):1160-72. doi: 10.1101/gad.1925210.

Macromolecules that prefer their membranes curvy.
Huang KC, Ramamurthi KS.
Mol Microbiol. 2010 May;76(4):822-32. doi: 10.1111/j.1365-2958.2010.07168.x. Epub 2010 Apr 25. Review.

2008

Cell shape and cell-wall organization in Gram-negative bacteria.
Huang KC, Mukhopadhyay R, Wen B, Gitai Z, Wingreen NS.
Proc Natl Acad Sci U S A. 2008 Dec 9;105(49):19282-7. doi: 10.1073/pnas.0805309105. Epub 2008 Dec 2.

Lipid localization in bacterial cells through curvature-mediated microphase separation.
Mukhopadhyay R*, Huang KC*, Wingreen NS.
Biophys J. 2008 Aug;95(3):1034-49. doi: 10.1529/biophysj.107.126920. Epub 2008 Apr 4.
*Co-first authors

The Min system as a general cell geometry detection mechanism: branch lengths in Y-shaped Escherichia coli cells affect Min oscillation patterns and division dynamics.
Varma A, Huang KC, Young KD.
J Bacteriol. 2008 Mar;190(6):2106-17. doi: 10.1128/JB.00720-07. Epub 2008 Jan 4.

2007

Cooperative gating and spatial organization of membrane proteins through elastic interactions.
Ursell T, Huang KC, Peterson E, Phillips R.
PLoS Comput Biol. 2007 May;3(5):e81.

Control of melting using nanoscale coatings
Huang KC, Wang T, and Joannopoulos JD.
Proceedings of The Minerals, Metals, and Materials Society (2007).

2006

A curvature-mediated mechanism for localization of lipids to bacterial poles.
Huang KC, Mukhopadhyay R, Wingreen NS.
PLoS Comput Biol. 2006 Nov 10;2(11):e151. Epub 2006 Oct 4.

2005

Nanoscale properties of melting at the surface of semiconductors
KC Huang, Wang T, and Joannopoulos JD.
Phys. Rev. B72 195314 (2005). Selected for November 21, 2005 issue of Virtual Journal of Nanoscale Science & Technology. 61.

Photonic Band-Gaps and Localization in the Thue-Morse Structures
X Jiang, Y Zhang, S Feng, KC Huang, Y Yi, and JD Joannopoulos.
Appl. Phys. Lett. 86 201110 (2005). Selected for May 23, 2005 issue of Virtual Journal of Nanoscale Science & Technology.

Superheating and induced melting at semiconductor interfaces.
Huang KC, Wang T, Joannopoulos JD.
Phys Rev Lett. 2005 May 6;94(17):175702. Epub 2005 May 3.

2004

Negative effective permeability in polaritonic photonic crystals
KC Huang, ML Povinelli, and JD Joannopoulos, Appl. Phys. Lett.85543 (2004). Selected for August 9, 2004 issue of Virtual Journal of Nanoscale Science & Technology

Min-protein oscillations in round bacteria.
Huang KC, Wingreen NS.
Phys Biol. 2004 Dec;1(3-4):229-35.

The nature of lossy Bloch states in polaritonic photonic crystals.
Huang KC, Lidorikis E, Jiang S, Joannopoulos JD, Nelson KA, Bienstman P, Fan S.
Phys Rev B. 2004 Jun 7; 69:195111. Epub 2004 Jun 7.

Pattern formation within Escherichia coli: diffusion, membrane attachment, and self-interaction of MinD molecules.
Kulkarni RV, Huang KC, Kloster M, Wingreen NS.
Phys Rev Lett. 2004 Nov 26;93(22):228103. Epub 2004 Nov 23.

2003

Dynamic structures in Escherichia coli: spontaneous formation of MinE rings and MinD polar zones.
Huang KC, Meir Y, Wingreen NS.
Proc Natl Acad Sci U S A. 2003 Oct 28;100(22):12724-8. Epub 2003 Oct 20.

Phonon-polariton excitations in photonic crystals.
Huang KC, Bienstman P, Joannopoulos JD, Nelson KA, Fan S.
Phys Rev B. 2003 Sept 8; 68:1075209. Epub 2003 Sept 8.

Field expulsion and reconfiguration in polaritonic photonic crystals.
Huang KC, Bienstman P, Joannopoulos JD, Nelson KA, Fan S.
Phys Rev Lett. 2003 May 16;90(19):196402. Epub 2003 May 13. Erratum in: Phys Rev Lett. 2004 Apr 23;92(16):169901.

2000

Comment on “Quantum Monte Carlo study of the dipole moment of CO” - J. Chem. Phys. 110, 11700 (1999)
Huang KC, Needs RJ, Rajagopal G.
J. Chem. Phys. 112, 4419 (2000).